Conveners
T1-1 Advances in Physics in Biology and Medicine Symp.: Protein system dynamics (DPMB) | Symposium sur les progrès en physique dans la biologie et la médecine: dynamique des systèmes de protéines (DPMB)
- Valerie Booth
Biological processes are stochastic reaction networks that operate far from thermodynamic equilibrium. Furthermore, even the best-known biological processes are not completely characterized in terms of mechanistic interactions between components. This combination makes analyzing noise properties challenging because small differences in rate functions or network topology can drastically affect...
Intrinsically disordered proteins (IDPs) play critical roles in regulatory protein interactions, but detailed structural/dynamics characterization of their ensembles remain challenging, both in isolation and they form dynamic ‘fuzzy’ complexes. Such is the case for mRNA cap-dependent translation initiation, which is regulated by the interaction of the predominantly folded eukaryotic initiation...
We report the results of experimental investigations involving photobiomodulation (PBM) of living cells, tubulin, and microtubules in buffer solutions exposed to near-infrared (NIR) light emitted from an $810$ nm LED with a power density of $25$ mW/cm$^2$ pulsed at a frequency of $10$ Hz. In the first group of experiments, we measured changes in the alternating current (AC) ionic conductivity...
Protein clustering occurs in living cells, often involving phase transitions, and can be an essential step in signal transduction in cells. Protein-protein interaction strength and diffusion times dictate when and how clusters occur, and with diffusion times dependent on geometric properties of cell compartments. The evolution of protein cluster sizes, and any signals sent by the clusters, can...
The 120-residue 4E-BP2 (BP2) protein undergoes a transition from disordered to partially folded upon multi-site phosphorylation, reducing its binding affinity with eIF4E (4E) and thus regulating the initiation of translation in neuronal cells. Although BP2 is an attractive target of anticancer drugs, its disordered nature makes it challenging to model. An initial ensemble of BP2 conformers...
A key challenge of systems biology is to translate cell heterogeneity data obtained from single-cell sequencing or flow cytometry experiments into causal and dynamic interactions. We show how static population snapshots of gene expression reporters can be used to infer causal and dynamic properties of gene regulatory networks without using perturbations. For instance, we derive correlation...
