Speaker
Marian Reincke
(University of Fribourg, Adolphe Merkle Institute)
Description
Solid state nanopores enable the analysis of single biomolecules in solution. Here we present a simple, in situ method for coating silicon nitride nanopores that addresses three of the main challenges in the field. By adding polymer directly to the recording buffer we were able to i) reduce non-specific interactions of the analyte with the pore; ii) suppress growth of the pore in electrolyte; iii) use nanopores on glass chips with superior noise properties for high bandwidth recordings.
Furthermore, experiments with aldolase show that the polymer des not affect the determination of protein volume.
Authors
Marian Reincke
(University of Fribourg, Adolphe Merkle Institute)
Dr
Saurabh Awasthi
(University of Fribourg, Adolphe Merkle Institute)
Prof.
Michael Mayer
(University of Fribourg, Adolphe Merkle Institute)