Conveners
(DPMB/DCMMP) T1-1 Soft Matter and Biological Physics Symposium | Symposium sur la matière molle et la physique biologique (DPMB/DPMCM)
- Maria Kilfoil (University of Prince Edward Island / Physics)
- Melanie Campbell
- Cornelia Hoehr
- Valerie Booth
- Robert Wickham
- Mamadou Diop (Western University & The Lawson Health Research Institute)
Description
Session I: Biopolymers in Confined Environments
I will present a unique quantitative single-molecule imaging platform called CLiC (Convex Lens-induced Confinement) which enables simultaneous measurements of the size, mRNA-payload, and dynamic properties of mRNA-based therapies and vaccines in controlled, cell-like conditions (Kamanzi et al, ACS Nano 2021). Here, we apply single-molecule biophysics to help characterize and understand the...
Even though dilute (unentangled) polymer solutions cannot act as gel-like sieving media, it has been shown that they can be used to separate DNA molecules in capillary electrophoresis. The separation then comes from sporadic and independent polyelectrolyte-polymer collisions. Here we explore such collisions in nanochannels (i.e., channels that are smaller than the normal size of the polymers),...
In recent years, nanofluidic devices have proven extremely useful for characterizing the physical behaviour of biopolymers such as DNA confined to narrow channels and micron-sized cavities. Insight gleaned from experiments using nanochannels is valuable for applications such as optical mapping of elongated DNA. Likewise, studies of multiple DNA molecules in nanocavities have provided insight...
