15-20 June 2014
Laurentian University / Université Laurentienne
America/Toronto timezone
Welcome to the 2014 CAP Congress! / Bienvenue au congrès de l'ACP 2014!

Evolution of the Min Protein Oscillation in *E. coli* Bacteria During Cell Growth and Division

18 Jun 2014, 14:15
15m
FA-054+5+6 (Laurentian University / Université Laurentienne)

FA-054+5+6

Laurentian University / Université Laurentienne

Sudbury, Ontario
Oral (Non-Student) / orale (non-étudiant) Medical and Biological Physics / Physique médicale et biologique (DMBP-DPMB) (W2-9) Molecular Biophysics - DMBP / Biophysique moléculaire - DPMB

Speaker

Dr Giuliani Maximiliano (University of Guelph)

Description

Cell division is a key step in the life of a bacterium. This process is carefully controlled and regulated so that the cellular machinery is equally partitioned into two daughter cells of equal size. In *E. coli*, this is accomplished, in part, by the Min protein system, in which Min proteins oscillate along the long axis of the rod-shaped cells. We have used high magnification, time-resolved fluorescence microscopy to characterize in detail the oscillation in *E. coli* cells in which the MinD proteins are tagged with green fluorescent protein (GFP). We have used a microfluidic device to confine the bacteria into microchannels that allows us to track the evolution of the oscillation in cells as they grow and divide in LB growth media. In particular, we have tracked the loss of synchrony between the oscillations in the daughter cells following cell division.

Primary author

Dr Giuliani Maximiliano (University of Guelph)

Co-authors

Mr Benjamin Baylis (University of Guelph) Dr John Dutcher (University of Guelph)

Presentation Materials

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