13-19 June 2015
University of Alberta
America/Edmonton timezone
Welcome to the 2015 CAP Congress! / Bienvenue au congrès de l'ACP 2015!

Evanescent Waveguide Microscopies for Bio-Application

16 Jun 2015, 16:45
30m
CCIS L2-200 (University of Alberta)

CCIS L2-200

University of Alberta

Invited Speaker / Conférencier invité Division of Atomic, Molecular and Optical Physics, Canada / Division de la physique atomique, moléculaire et photonique, Canada (DAMOPC-DPAMPC) T3-2 Quantum Computation and Communication (DTP-DCMMP-DAMOPC) / Communication et calcul quantique (DPT-DPMCM-DPAMPC)

Speaker

Prof. Silvia Mittler (University of Western Ontario)

Description

Two new evanescent field microscopy technologies based on glass slab waveguides with permanent coupling gratings are introduced: waveguide evanescent field fluorescence (WEFF) microscopy and waveguide evanescent field scattering (WEFS) microscopy. The technologies are briefly described and the experimental setup based on a conventional inverted microscope is introduced and compared to existing technologies like TIR and TIRF. The advantages over the existing technologies are clearly addressed. For each technology one application in cell biology is shown. With multimode WEFF microscopy, taking at least two images with two different waveguide modes, it is possible to determine the fluorescence dye location above the waveguide surface. Therefore 2D dye distance maps or 3D contour plots can be calculated for the samples. As an example, the bending of the plasma membranes of cells between focal adhesions and focal contacts to the waveguide surface are investigated. WEFS microscopy which works as a label-free microscopy is used to analyse bacterial biofilm formation: from a parent cell to micro-colonies. In addition experiments on bacterial UV sterilization and its consequences on biofilm formation are shown.

Primary author

Prof. Silvia Mittler (University of Western Ontario)

Presentation Materials

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