Jun 13 – 19, 2015
University of Alberta
America/Edmonton timezone
Welcome to the 2015 CAP Congress! / Bienvenue au congrès de l'ACP 2015!

Getting into that "Room at the Bottom" of DNA Analysis using Tunable Nanoscale Confinement

Jun 18, 2015, 4:15 PM
CCIS 1-140 (University of Alberta)

CCIS 1-140

University of Alberta

Invited Speaker / Conférencier invité Condensed Matter and Materials Physics / Physique de la matière condensée et matériaux (DCMMP-DPMCM) R3-2 Polymers and Biopolymers (DCMMP-DMBP) / Polymères et biopolymères (DPMCM-DPMB)


Sabrina Leslie (McGill University)


A wide range of life-preserving processes, such as DNA transcription and repair, rely on weak intermolecular interactions and slow dynamics which occur at high concentrations, over long time periods, and often under confinement. Visualizing dynamic processes can present a challenge to fluorescence microscopy, the work horse for resolving biological processes at the molecular scale. To address this challenge, we present new in vitro diagnostics which use tunable and transverse nanoscale confinement to bring biomolecules into crisp view under previously inaccessible conditions, approaching those in the human body. We tackle the challenge of manipulating and visualizing long strands of DNA for genomic analysis, ultimately extracted from single cells (Berard et al, PNAS 2014 and Commentary). Further, we develop nanoscale spectroscopy methods to establish how conformational DNA fluctuations regulate transcription and gene expression, which remains an open and compelling question.

Primary author

Sabrina Leslie (McGill University)

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