8-10 June 2016
Asia/Bangkok timezone

Development of mobile photometer for DNA quantitation using UV-LED.

Jun 8, 2016, 11:30 AM
15m
Room E1

Room E1

Oral presentaion Instrumentation, Metrology and Standards Session V

Speaker

Kathawut Thamthinthai (Mahidol University)

Description

Light as electromagnetic wave when travelling through a liquid solution, it could perturb and interact with molecules in that solution. DNA absorbs light specifically at 260 nm. The absorbance is related to DNA concentration through Beer-Lambert law of absorption. This relationship is a common basis of DNA quantification in spectrophotometers. A bulky deuterium lamp or halogen lamp is commonly used as 260-nm light source. Their use has limited the mobility of spectrophotometer. UV-LED is an alternative light source with light weight and more compact in size. In this work, we have developed 260-nm LED and a photo-diode as a compact and mobile photometer. At present, the development is still in its early stage. Some experimental results of DNA quantification are reported in this conference.

Primary author

Kathawut Thamthinthai (Mahidol University)

Co-author

Somsak Dangtip (Mahidol University)

Presentation materials

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