Hydroxyapatite, Ca10(PO4)6(OH)2 were prepared by the reaction of calcium hydroxide from waste chicken eggshell and di-ammonium hydrogen orthophosphate solution and heated at different temperature from 200 to 700 °C for 4 hour. The crystal structure, function group and morphology of hydroxyapatite were investigated by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), respectively. The structure was found to be hydroxyapatite phase at 200 to 600°C and the crystalline size increased with increasing temperature. However, the hydroxyapatite phase was transformed to tri-calcium phosphate phase completely at 700 °C. The morphology of hydroxyapatite were agglomerates and sphere particles. These experiments show that the hydroxyapatite could be synthesized from waste chicken eggshell and reduced time and cost for biomaterials application.