28 May 2017 to 2 June 2017
Queen's University
America/Toronto timezone
Welcome to the 2017 CAP Congress! / Bienvenue au congrès de l'ACP 2017!

A dual-docking microfluidic cell migration assay for testing neutrophil chemotaxis and the memory effect

30 May 2017, 16:45
15m
Miller Hall 105 (Queen's University)

Miller Hall 105

Queen's University

CLOSED - Oral (Non-Student) / orale (non-étudiant) Physics in Medicine and Biology / Physique en médecine et en biologie (DPMB-DPMB) T4-7 Biomechanics and Fluid Dynamics (DPMB) | Biomécanique et dynamique des fluides (DPMB)

Speaker

Prof. Francis Lin (University of Manitoba)

Description

Chemotaxis is a classic mechanism for guiding cell migration and an important topic in both fundamental cell biology and health science. Neutrophil is a widely used model to study eukaryotic cell migration and neutrophil chemotaxis itself can lead to protective or harmful immune actions to the body. While much has been learnt from past research about how neutrophils effectively navigate through a chemoattractant gradient, many interesting questions remain unclear. For example, while it is tempting to model neutrophil chemotaxis using the well-established biased random walk theory, the experimental proof was challenged by the cell’s highly persistent migration nature. Special experimental design is required to test the key predictions from the random walk model. Another question that interests the cell migration community for decades concerns the existence of chemotactic memory and its underlying mechanism. Although chemotactic memory has been suggested in various studies, a clear quantitative experimental demonstration will improve our understanding of the migratory memory effect. Motivated by these questions, we developed a microfluidic cell migration assay (so-called dual-docking chip or D2-Chip) that can test both the biased random walk model and the memory effect for neutrophil chemotaxis on a single chip enabled by multi-region gradient generation and dual-region cell alignment. Our results provided evidence to support the biased random walk as a possible mechanism for neutrophil chemotaxis and clearly demonstrated chemotactic memory. In summary, this work highlights the possible biophysical mechanism employed by neutrophils for directional migratory response to their guiding chemical microenvironment and integrates the microfluidics approach to address cell migration questions.

Primary authors

Mr Ke Yang (University of Manitoba; Chinese Academy of Sciences) Ms Dongxue Xie (University of Manitoba; Jilin University) Dr Hagit Peretz-Soroka (University of Manitoba) Dr Susy Santos (South Winnipeg Integrated Health & Social Services) Prof. Murray Alexander (University of Winnipeg) Dr Ling Zhu (Chinese Academy of Sciences) Dr Michael Zhang (Seven Oaks General Hospital (Winnipeg)) Dr Yong Liu (Chinese Academy of Sciences) Prof. Francis Lin (University of Manitoba) Mr Jiandong Wu (University of Manitoba) Mr Guoqing Xu (University of Manitoba; University of Winnipeg)

Presentation Materials

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