10-16 June 2018
Dalhousie University
America/Halifax timezone
Welcome to the 2018 CAP Congress Program website! / Bienvenue au siteweb du programme du Congrès de l'ACP 2018!

POS-51 Bacterial Mounting and Concentration Techniques to Translate Laser-Induced Breakdown Spectroscopy into a Clinical Setting

12 Jun 2018, 18:00
1h 30m
SUB McInnes Hall (Dalhousie University)

SUB McInnes Hall

Dalhousie University

Poster (Graduate Student) / Affiche (Étudiant(e) du 2e ou 3e cycle) Physics in Medicine and Biology / Physique en médecine et en biologie (DPMB-DPMB) DPMB Poster Session & Finals: Poster competition and Mingle session with Industrial partners/employers (9) | Session d'affiches DPMB et finales: Concours d'affiches et rencontres avec partenaires industriels et employeurs (9)

Speaker

Ms Alexandra Paulick (University of Windsor)

Description

Laser-induced breakdown spectroscopy (LIBS) is a rapid elemental analysis technique that has been used for the detection and identification of bacterial pathogens. There is a high demand for real-time identification of bacteria and the capabilities of LIBS for this are promising. Optimizing the bacterial mounting techniques prior to LIBS analysis in a clinical setting is currently underway. This includes the sample preparation steps of separating bacterial cells from the other unwanted cells that would most likely be present in a biological specimen. It also includes lowering the minimum number of bacterial cells required for detection and accurate identification by LIBS.

This poster will present our efforts to accomplish these goals. We have investigated the effectiveness of the detergent Tween 20 as a possible anti-clumping agent for bacterial cells deposited on a nitrocellulose filter paper by observing the LIBS signal from E. coli specimens deposited both with and without Tween. The ability to quickly separate a contaminant from a bacterial suspension through the use of centrifugation and filter media with different pore sizes will also be presented. In addition, the capability of a metal cone designed to concentrate bacterial depositions during centrifugation onto a smaller area of the filter paper will be demonstrated.

Primary author

Ms Alexandra Paulick (University of Windsor)

Co-authors

Ms Naila Rahman (University of Windsor) Dr Steven Rehse (University of Windsor)

Presentation Materials

There are no materials yet.