Xylanase enzyme families play an important role in many industries due to its ability to digest the hemicellulose within plant cell walls. Developing the enzyme that can withstand extreme conditions under an industrial process and in a stomach requires an extended information in molecular scale for further protein engineering. In this study, atomistic molecular dynamics simulation and analysis...
One of the interesting methods used in disrupting microalgae is ultrasonication. In this study, the Keller-Miksis equation was used to simulate the dynamics of cavitation bubble inside microalgae cell species C. gracilis, C. calcitrans and Nannochloropsis sp. at frequencies of 1.0, 2.2, 3.3 and 4.3 MHz at the same acoustic intensity of 2.2 W/cm$^2$ and the physical effects of collpased...
Electroporation is the technique used in creating pores on cell membrane by exposing the cell to high electric field strength. Microfluidic electroporation device takes advantages of miniaturized fluidic channel and electrode fabrication in obtaining high electric field strength using low applied voltage. In this work, the tapered microfluidic device for E. coli electroporation was developed...
In both electro-permeabilization and electrofusion, a high-intensity DC pulse electric field is employed to induced membrane breakdown and deliver macromolecules into living- targeted biological cells. The transient electric field is always applied in a pulsed form to prevent irreversible cell damage. The most commonly used waveforms are the rectangular pulse (square pulse, SP) and the...
This research had been extended from our previous project of electrical sex reversal for monosex-male Nile tilapia by using pulse-electric fields (Fish X-Change) to increase all-male sex reversal (MSR), survival rate (SR), hatching rate (HR) and cleaning cell surfaces during the hatchery process. In order to reduce the mortality rate and increase the survival rate, the effect of high-voltage...
Present work to investigation of structure and functional properties of nano materials based on the Titanium dioxide doping Selenium oxide and Turmeric coated cellulose fibers of gauze pad cotton. The Titania hydrosol was successfully prepared at low temperature by a microwave facilitated sol-gel method. The study also explored the efficiency of this sample to inhibit the growth of Escherichia...
Diverse morphological shapes of cyanobacteria (blue green algae) have been observed in the natural environment; Synechocystis sp is found to be spherical, whereas Arthrospira is either a rod in a stationary phase or a helix in a logarithmic growth phase. Effects of Reynolds number and shapes on the localized drag exerted on solid objects with different geometrical shapes resembling the shapes...
Experimental facts have showed that in application of low-energy ion beam for biological living materials modification, the ion beam fluence required to induce cell mutation is orders higher than the cell lethal dose. This seems contradictory with common perception that the DNA modification should be proportional to radiation dose so that high dose radiation could cause high-degree damage in...