Speaker
Description
Transcription factors (TFs) organize within the nucleus in clusters. These clusters play a crucial role in regulating transcription activity. In zebrafish, two large Nanog clusters form soon after fertilization. Nanog proteins possess a DNA-binding-domain, for proper condensate assembly, alongside with two disordered domains, facilitating protein-protein interactions. However, the contributions of these domains remain elusive.
Here, we propose a quantitative study to investigate Nanog dynamics using microscopy techniques. Through FCS, we will elucidate Nanog mobility, while, FRET will enable to measure Nanog-chromatin interactions, providing insights into gene expression regulation.
We aim to create a model describing the contributions of distinct Nanog domains to condensate dynamics, advancing our understanding of transcription activity.
