Speaker
Description
Respiratory chain super-complexes (SC) are naturally occurring assemblies of oxidative phosphorylation protein complexes. Their role in facilitating mitochondrial ATP production remains debated. While altered metabolic conditions affect SC occurrence and isolated SC have been structurally characterized, their spatial organization and stoichiometry within mitochondria remain unclear. In this study, we employed expansion microscopy (ExM) combined with stimulated emission depletion microscopy (STED) to resolve the individual protein complexes that together form SCs in human cells. Our results provide novel insights into the organization and stoichiometry of SCs in situ, allowing us to propose hypotheses regarding their functional impact on mitochondrial physiology.
