Speaker
Vojislav Gligorovski
(Laboratory of the Physics of Biological Systems, Institute of Physics, EPFL)
Description
Existing approaches in directed evolution are suited for evolving steady-state properties such as enzymatic activity or protein binding. A fundamental problem remains how to evolve dynamic proteins. As a solution, we present coupling such proteins to a dynamic system essential for cell survival. We first evolved mutants of a LOV transcription factor that were stronger, less leaky, or red-responsive. Evolving the PhyB-Pif3 dimerization system, we discovered mutations that make the exogenous chromophore unnecessary. Finally, to demonstrate the generality of the method, we evolved an AND gate with a chemical input. Our method represents new paradigm for evolution of dynamic proteins.
Author
Vojislav Gligorovski
(Laboratory of the Physics of Biological Systems, Institute of Physics, EPFL)
