Speaker
Philipp Cedro
(Roche)
Description
Focal Molography is a new label-free method for real-time molecular interaction analysis in buffers, as well as in complex media, such as cell lysate. Here, we show an applied project from drug discovery research of an interaction between a protein and a peptide. The kinetic rate constant (KD) of the interaction was determined in buffer and in cell lysate (1 Mio cells / mL). We have shown that Focal Molography is able to reproducibly measure the KD without any interference of non-specific binding. This new tool will lift drug discovery to new levels of interaction studies.
Author
Philipp Cedro
(Roche)
