Speaker
Ms
Ya-Fang Chang
(Institute of Radiological Sciences, National Yang-Ming University, Taipei, Taiwan)
Description
Recently, molecular imaging of reporter gene expression provides a rapid,
sensitive, and noninvasive monitoring of tumor behaviors. In this study, we
reported the establishment of novel animal models using immunodeficient mice for
longitudinal examination of tumor growth kinetics and metastatic spreading in vivo.
The highly metastatic MDA-MB-435s cell line was engineered to stably expressed
herpes simplex virus type 1 thymidine kinase (HSV-1-tk) and luciferase. Both 131I-
FIAU and D-luciferin were used as probes. For orthotopic tumor formation, MDA-MB-
435s-tk/luc cells were implanted into the first nipple of 6-week-old female
NOD/SCID mice. For metastatic study, cells were injected via the lateral tail vein.
Mice bearing MDA-MB-435s-tk/luc tumors were imaged for tumor growth and metastatic
sites using Xenogen IVIS50 bioluminescent system. Gamma scintigraphy, whole-body
autoradiography were also applied to confirm the localization. The result of
bioluminescent imaging showed that distinct photon accumulation could be detected
and quantified in the femur, spine, spleen, salivary gland, lymph nodes, and muscle
at 16 weeks post i.v. injection. Histopathological examination further verified
tumor metastasis at those organs as well. We concluded that establishment of MDA-MB-
435s-tk/luc human breast carcinoma-bearing mouse model combined with
multimodalities of molecular imaging can facilitate studies on the molecular
mechanisms behind invasion and metastasis, and also allow a more convenient,
sensitive and noninvasive imaging protocol for the diagnosis of the diseases as
well as for the evaluation of therapeutic efficacy of newly developed drugs or new
treatment trial in preclinical studies.
Authors
Prof.
Jeng-Jong Hwang
(Institute of Radiological Sciences, National Yang-Ming University, Taipei, Taiwan)
Ms
Ya-Fang Chang
(Institute of Radiological Sciences, National Yang-Ming University, Taipei, Taiwan)
Co-authors
Prof.
Fei Pang
(Dept. of Veterinary Medicine, National Taiwan University, Taipei, Taiwan)
Prof.
Hsin-Ell Wang
(Institute of Radiological Sciences, National Yang-Ming University, Taipei, Taiwan)
Dr
Ren-Shen Liu
(Dept. of Nuclear Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan)
Mr
Yi-Yu Lin
(Institute of Radiological Sciences, National Yang-Ming University, Taipei, Taiwan)
