Euromedim 2006 :1st European Conference on Molecular Imaging Technology

188 RHENIUM-INDUCED CELL DEATH AND APOPTOSIS IN A PANEL OF TUMOR CELL LINES

11 May 2006, 14:00

1h

Palais du Pharo, Marseille

poster Molecular Imaging needs for biologists and physicians Poster session : Imaging systems, Molecular Imaging

Speaker

Dr Andrea Perrotta (INFN,Dipartimento di Fisica, Bologna)

Description

Rhenium-188 (188Re) represents an interesting radioisotope in cancer therapy for its β- radiation of high energy and γ-ray useful in scintigraphic applications. The genotoxic effect of 188Re-perrhenate has been evaluated on a panel of tumour cell lines (U87,H460,MCF-7,LNCaP,A549,M5076) established from tumors of different type (glioblstoma, lung, breast and prostate cancer) in terms of inhibition of cell viability, micronuclei (MN) and apoptosis. Cells were treated with different activities of 188Re-perrhenate (50-200 μCi) and harvested 48-72 hours later for MTT staining. All the tested cell lines established from lung, breast and prostate cancer seemed to be particularly sensitive to treatment except U87 glioblastoma cells, which behaved as radioresistant. On the contrary, micronuclei induction in binucleated cells was significantly higher in the U87 glioblastoma cells compared to LNCaP and H460 after 48 hours exposure to 25-100 μCi. Induction of apoptosis assessed by apoptotic bodies or TUNEL staining, showed that MTT radioresistance observed in U87 cells correlated well with a very low extent of apoptosis. These results suggest that the evaluation of apoptosis induction rather than induction of MN is a useful tool to asses resistance/sensitivity to treatment with 188Re. In addition, these preliminary results indicate that as a next step radiopharmaceuticals containing 188Re may be successfully used to target lung, breast and prostate tumors in “in vivo” mouse system. ACKNOWLEDGEMENTS Supported by Gruppo V, INFN, Italy.