Speaker
Ms
Anne Osdoit
(MKT)
Description
The development of the Red Cell~vizio, using a red 635 nm laser, makes it possible to
obtain microscopic in vivo images even with the large range of red fluorescent dyes
that are widely used in the field of molecular imaging.
The Cell~vizio™, based on Fibered Confocal Fluorescence Microscopy, enables in vivo
and in situ microscopy down to a resolution of 2.5 µm. Until now, the Cell~vizio™
was only available with a blue 488 nm laser catering to the range of fluorophores and
transgenic animals that have an emission bandwidth of 500 to 650 nm. The
introduction of the Red Cell~vizio now offers the possibility to cover a large number
of fluorophores like Cy5.5 or animal models that emit fluorescent signal between 650
and 750 nm. Such wavelengths are being widely used in whole body optical imaging
techniques and hence the Red Cell~vizio allows the use of the same models to obtain
microscopic in vivo real-time images.
The Cell~vizio is a stand-alone imaging system made of a Laser Scanning Unit, a range
of fibered objectives made of tens of thousands of optical fibers (the “ProFlex”) and
the ImageCell image processing and analysis software. High definition images with
2.5 µm lateral resolution, 15 to 20 µm axial resolution, and a field of-view up to
600 µm x 500 µm can be obtained at a rate of 12 frames per second on living
anesthetized animals. These images can be obtained at depths up to 100 µm. The
fibered probes have diameters ranging from 300 µm to 1.8 mm and thus offer unique
access capabilities.
The Cell~vizio has been applied to scientific research across the world in the areas
of peripheral nerve studies, deep brain imaging, microcirculation, angiogenesis,
immunology, oncology and ophthalmology. Convincing results and images were obtained
in those fields.
Author
Ms
Anne Osdoit
(MKT)
Co-authors
Mr
Benjamin Abrat
(Mauna Kea Technologies)
Mr
Bruno Brisson
(Mauna Kea Technologies)
Ms
Charlotte Cavé
(Mauna Kea Technologies)
Mr
Sacha Loiseau
(Mauna Kea Technologies)
