Speaker
Description
In this work, we present a new electrochemical DNA biosensor using screen-printed
graphene electrode and Hoechst 33258 intercalator for detection of Aspergillus flavas
DNA that produces aflatoxin B1. The principle of detection is based on suppression
of activity of redox molecules (H33258), which will be intercalated between A-T
based of hybridized DNA of Aspergillus flavas that produces aflatoxin B1, resulting
in a decrease of anodic current (∆I). There are five processes including DNA
extraction, DNA amplification, hybridization, mediator mixing and electrochemical
detection. For electrochemical detection, the detected current will be converted to
aflatoxin concentration within 20 s. The measured value will be shown on LED
display. A good linear relationship in obtained over concentration range of 0 to 400
ppb. The developed AflaSense was applied to successfully determine aflatoxin in 52
samples.
