Speaker
Oliver Zerbe
Description
I will report on our developments of methodology for assigning 7-TM membrane proteins by solution NMR methods. To this end, information from triple-resonance NMR spectra, from 3D and 4D NOESY spectra and from biochemical and topological information is fed as restraints into the FLYA module of CYANA. Thereby we could assignment more than 60 % of backbone and methyl groups of bacteriorhodopsin incorporated into lipid nanodiscs without mutagenesis. In addition, we probed dynamics of methyl groups.
Furthermore, I present first results of our NMR studies conducted on the a1b adrenergic receptor. I will in particular highlight our efforts to produce receptor constructs stable for NMR measurements at elevated temperature.
Author
Oliver Zerbe
