XXIII Swiss NMR Symposium

Session

NMR session 2

14 Jan 2020, 11:30

503/1-001 - Council Chamber (CERN)

8. Real-time monitoring of metabolism and mitochondrial respiration in 3D cell culture systems by NMR

Peter Vermathen (DRNN & DBMR, University Bern)

14/01/2020, 11:30

The feasibility to determine metabolism and oxygen consumption of living 3D cell culture systems by NMR in standard 5mm tubes is demonstrated. High cell viability was obtained and the measurements were stable and reproducible over >12 hours. We describe the effect of the flow rate on metabolic activity. The sensitivity to detect substrate degradation rates of major mitochondrial fuel pathways...

18. New tools to study protein phase separation by solution NMR

Leonidas Emmanouilidis

14/01/2020, 11:45

10. Biomolecular mechanisms unraveled by solution NMR spectroscopy

Sebastian Hiller (Universität Basel)

14/01/2020, 12:00

Solution NMR spectroscopy is a matured technique to study structure, function, and dynamics of biomacromolecules at atomic resolution. Of particular value in the current era of structural biology are integrative approaches to combine data from multiple structure biology techniques to integrated molecular models. I will give an overview of ongoing projects in my laboratory at Biozentrum Basel...

3. NMR tools for studying large (>100 kDa) protonated proteins produced in human cells

Dr Alvar Gossert (ETH Zürich)

14/01/2020, 12:15

Our aim is to study proteins that are relevant for human diseases at atomic resolution. Many of the important drug target proteins – like G-protein coupled receptors – are difficult to study by NMR in solution, as they can often only be produced in eukaryotic expression hosts and are larger than 100 kDa.
We have therefore developed protocols for isotope labeling in eukaryotic cell...

41. Multiple states of proteins in vitro and in cells

Roland Riek (ETHZ - ETH Zurich)

14/01/2020, 12:30

The 3D structure and dynamics of proteins are key of their functions. Proteins undergo thereby structural changes from one state to another one.
With the advent of exact NOEs structural ensembles of proteins are determined at atomic resolution highlighting this structure-dynamic mechanism in the case of enzymes and protein allostery in vitro. In parallel, in cell NMR shows the presence of...

23. Intercepting c-di-GMP signaling by a c-di-GMP sequestering peptide

Judith Habazettl

14/01/2020, 12:45

22. NMR spectroscopy of RNA G-quadruplexes

Silke Johannsen

14/01/2020, 13:00

As one kind of non-canonical structures of nucleic acid, G-quadruplexes (G4) exist universally in the
genome of different organisms and in humans. G4 are supposed to play crucial regulatory roles in gene
replication, translation and expression. Therefore, G4s are handled as a promising target for chemotherapy. To date, most studies concentrated on DNA G4s, while the understanding of RNA G4s...