The feasibility to determine metabolism and oxygen consumption of living 3D cell culture systems by NMR in standard 5mm tubes is demonstrated. High cell viability was obtained and the measurements were stable and reproducible over >12 hours. We describe the effect of the flow rate on metabolic activity. The sensitivity to detect substrate degradation rates of major mitochondrial fuel pathways...
Solution NMR spectroscopy is a matured technique to study structure, function, and dynamics of biomacromolecules at atomic resolution. Of particular value in the current era of structural biology are integrative approaches to combine data from multiple structure biology techniques to integrated molecular models. I will give an overview of ongoing projects in my laboratory at Biozentrum Basel...
Our aim is to study proteins that are relevant for human diseases at atomic resolution. Many of the important drug target proteins – like G-protein coupled receptors – are difficult to study by NMR in solution, as they can often only be produced in eukaryotic expression hosts and are larger than 100 kDa.
We have therefore developed protocols for isotope labeling in eukaryotic cell...
The 3D structure and dynamics of proteins are key of their functions. Proteins undergo thereby structural changes from one state to another one.
With the advent of exact NOEs structural ensembles of proteins are determined at atomic resolution highlighting this structure-dynamic mechanism in the case of enzymes and protein allostery in vitro. In parallel, in cell NMR shows the presence of...