Speakers
Prof.
Chenguo Yao
(Chongqing University)
Yajun Zhao
(Chongqing University)
Description
Muscle contraction and the non-uniform distribution have become the key obstacles which restrict the development of the irreversible electroporation in clinical application. To solve these two issues, a novel type of pulses called high-frequency composite pulses was proposed by our group. The killing effect and the thermal effect were investigated by our homemade pulse generator. The high-frequency composite pulses with different amplitudes and inner frequency of burst were used to treat the human melanoma cells (Gll19) and the cell viability and the temperature rising under different parameters were evaluated. The results showed that, the cell viability would decrease with the increase of the electrical field amplitude (1kV/cm-2.5kV/cm), and the change of the temperature (4 oC -40 oC) was square of the amplitude. However, the inner frequency of burst had little effect on the temperature rising, and the cell viability had a slight decrease with the increase of the frequency. Therefore, the appropriate electric field strength should be selected to balance the killing effect and the thermal effect, while the inner frequency of the burst in the certain range have insignificant effect on both the cell viability and temperature rising. According to the requirement of the uniform electric field and the inhabitation of the muscle contraction, the optimal inner frequency with appropriate frequency content should be determined. This study revealed the relationship between the high-frequency composite pulse parameters and the cell viability and the thermal effect which was meaningful for the further investigation of the novel type of pulses.
Primary author
Yajun Zhao
(Chongqing University)
Co-authors
Prof.
Chenguo Yao
(Chongqing University)
Dr
Chengxiang Li
(Chongqing University)
Mrs
Hongmei Liu
(Chongqing University)
Mr
Shoulog Dong
(Chongqing University)
Dr
Yan Mi
(Chongqing University)
Mr
Yanpeng Lv
(Chongqing University)
