Speaker
Gabriel Campargue
(GAP-Biophotonics, University of Geneva, Switzerland)
Description
In femtosecond optical transfection and nanosurgery, cell survival critically depends on phototoxicity. We demonstrated on fixed cells that the use of femtosecond third order phase pulses (Airy pulses) could modulate the photon-interaction in cells leading to a better membrane poration efficiency energy per pulse than bandwith limited femtosecond pulses.
We also discuss results from a live cell experiment. We set up a protocol where we investigate cell viability as a function of pulse spectral phase. Hence, we present an ad hoc protocol based on the use of three fluorophores to assess cells viability at different time points after poration.
Authors
Gabriel Campargue
(GAP-Biophotonics, University of Geneva, Switzerland)
Bastian Zielinski
(University of Kassel, Institute of Physics, Germany)
Dr
Sébastien Courvoisier
(GAP-Biophotonics, University of Geneva, Switzerland)
Cristian Sarpe
(University of Kassel, Institute of Physics, Germany)
Dr
Luigi Bonacina
(GAP-Biophotonics, University of Geneva, Switzerland)
Prof.
Thomas Baumert
(University of Kassel, Institute of Physics, Germany)
Prof.
Jean-Pierre Wolf
(GAP-Biophotonics, University of Geneva, Switzerland)
