Speaker
Peter Vermathen
(DRNN & DBMR, University Bern)
Description
The feasibility to determine metabolism and oxygen consumption of living 3D cell culture systems by NMR in standard 5mm tubes is demonstrated. High cell viability was obtained and the measurements were stable and reproducible over >12 hours. We describe the effect of the flow rate on metabolic activity. The sensitivity to detect substrate degradation rates of major mitochondrial fuel pathways is demonstrated and the ability to measure rapid O2 and lactate changes as surrogate markers of oxidative phosphorylation and anaerobic glycolysis.
Authors
Dr
Andrea Felser
(Institute of Clinical Chemistry, University Hospital Bern)
Mr
Damian Hertig
(Institute of Clinical Chemistry, University Hospital Bern)
Dr
Jean-Marc Nuoffer
(Institute of Clinical Chemistry, University Hospital Bern)
Peter Vermathen
(DRNN & DBMR, University Bern)
Mrs
Sally Maddah
(DRNN & DBMR, University Bern)
