Speaker
Description
Raman (RS) and infrared (IR) spectroscopy are used as versatile tools for examination of biological systems, that can be conducted in two ways – in a label-free or labelled manner. The second approach uses specific chemical compounds, called spectroscopic reporters. They contain chemical moieties (alkyne, nitrile, or C-D bonds) that are active in Raman and/or IR and exhibit bands in the so-called silent region. Spectroscopic tags, due to the special chemical group, bind to a target organelle or enter specific metabolic pathways.
In our studies, we developed the library of spectroscopic reporters by performing molecular characterization (RS, IR and partially UV) of the most promising compounds for further applications in bio-imaging: EdU (nucleus); MitoBADY (mitochondria); dPA and dOA (lipids metabolism); 3-OPG and d7-glucose (glucose metabolism). The proposed library of reporters is a convenient set of targeting molecules that allow for a detailed in vitro examination of metabolic processes with development potential.
This research was funded in whole or in part by the National Science Center Poland (NCN), Maestro 2022/46/A/ST4/00054 to Malgorzata Baranska.
| Field | Chemistry |
|---|---|
| Length | Short 15 min |