Mr
Kacper Stawoski
(Jagiellonian University in Kraków, Faculty of Chemistry, Department of Chemical Physics, Kraków, Poland)
Acute lymphoblastic leukemia (ALL) is caused by an abnormal proliferation of immature B-cells or T-cells and occurs mainly in children.[1] Among various subtypes of ALL, some are characterized by the overactivation of the JAK-STAT signaling pathway. In such cases, treatment may include JAK inhibitors e.g. ruxolitinib.[2] The aim of this study was to evaluate the biochemical changes of B-ALL cells, treated with ruxolitinib, with the use of Raman imaging paired with machine learning statistical analysis methods. We used in vitro models of B-ALL cell lines that harbor (MUTZ5) or lack JAK mutation (SEM). Spectroscopic markers of biochemical changes were defined with the use of supervised (PLS) and unsupervised (PCA) chemometric analysis methods. MUTZ5 cells treated with ruxolitinib showed an increased content of lipidic structures and proteins compared to their untreated counterparts, while no similar effect was observed for SEM cells.
The „Label-free and rapid optical imaging, detection and sorting of leukemia cells” project is carried out within the Team-Net programme of the Foundation for Polish Science co-financed by the EU.
| Field |
Chemistry
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| Length |
Short 15 min
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Mr
Kacper Stawoski
(Jagiellonian University in Kraków, Faculty of Chemistry, Department of Chemical Physics, Kraków, Poland)
Dr
Adrianna Adamczyk
(Jagiellonian University in Kraków, Faculty of Chemistry, Department of Chemical Physics, Kraków, Poland)
Dr
Anna M. Nowakowska
(Jagiellonian University in Kraków, Faculty of Chemistry, Department of Chemical Physics, Kraków, Poland)
Dr
Justyna Jakubowska
(Department of Pediatrics, Oncology and Hematology, Medical University of Łódź, Łódź, Poland)
Dr
Marta Ząbczyńska
(Department of Pediatrics, Oncology and Hematology, Medical University of Łódź, Łódź, Poland)
Dr
Agata Pastorczak
(Department of Pediatrics, Oncology and Hematology, Medical University of Łódź, Łódź, Poland)
Dr
Kinga Ostrowska
(Department of Pediatrics, Oncology and Hematology, Medical University of Łódź, Łódź, Poland)
Prof.
Wojciech Młynarski
(Department of Pediatrics, Oncology and Hematology, Medical University of Łódź, Łódź, Poland)
Dr
Katarzyna Majzner
(Jagiellonian University in Kraków, Faculty of Chemistry, Department of Chemical Physics, Kraków, Poland)
Prof.
Małgorzata Barańska
(1.Jagiellonian University in Kraków, Faculty of Chemistry, Department of Chemical Physics, Kraków, Poland, 2.Jagiellonian University in Kraków, Jagiellonian Centre for Experimental Therapeutics (JCET), Kraków, Poland)
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