Speaker
Description
Raman scattering is mainly associated with label-free bioimaging, but the presence of specific measurement and experimental conditions may significantly increase the intensity of registered signals, and therefore image quality. One way to enhance the signal coming from the subcellular structure of choice is the introduction of a special probe molecule into the cell, which exhibits different oscillations from those of endogenous biomolecules. In this case, bands specific for the reporter are usually observed in the so-called silent region of the Raman spectrum. Another approach includes taking advantage of the energy transfer mechanism to enhance the signal of substances that can be normally found in the cell.
Preliminary research of near field energy transfer was carried out using stimulated Raman scattering (SRS). A group of dyes dissolved in dimethyl sulfoxide (DMSO) was studied. The studies focused on comparing stimulated Raman loss (SRL) values at 2766 cm$^{-1}$ and 3100 cm$^{-1}$ where DMSO does not absorb energy with the SRL signal at 2916 cm$^{-1}$ and 3001 cm$^{-1}$ where the DMSO absorption bands are located. A comparison between pure DMSO and DMSO dye solutions was made.
| Field | Chemistry |
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| Length | Short 15 min |