Speaker
Description
Glioblastoma, ranked among the most aggressive types of neoplasms of the central nervous system, is characterized by very high mortality rate. The understanding of such processes constitutes a key step in the development of innovative, effective strategies of treatment to improve the prognosis of this devastating condition.
Three different glioma cell lines were chosen–human LN229, U87 and murine GL261 and microscopic images were taken continually during the cell growth (for the period of one week, using JuLI™ Stage Real-Time CHR). Using advanced imaging analysis, cells were automatically counted and their morphology was analyzed.
The automatic collection of images during growth enabled continuous monitoring of cell proliferation and migration. In a hypoxic environment, cells exhibit a greater degree of elongation and enhanced substrate adhesion, thus categorizing them as more flattened in morphology. This analysis reveals subtle differences in the rates of cell proliferation among the examined cell lines. LN229 and U87 exhibit comparatively shorter doubling times than GL261, implying a potential for higher proliferative activity within these specific lines.
| Field | Biosciences |
|---|---|
| Length | Quick 10 min |
