Jirattiporn Sanguansuttigul, Kheamrutai Thamaphat* and Pichet Limsuwan
Green Synthesis and Application Laboratory, Applied Science and Engineering for Social Solution,
Department of Physics, Faculty of Science, King Mongkut’s University of Technology Thonburi, Bangmod, Thungkru, Bangkok 10140
In food, beverage, and sugar production processes, one of the most important control parameters required in the product quality control is sucrose content or named as Brix percentage. Several methods have been developed to measure the Brix percentage. The UV-Vis spectroscopy is an alternative method to analysis sucrose content in solution due to its easy and simple. Based on Beer-Lambert law, the results showed that the highest absorption peak of sucrose solution was observed at approximately 197 nm associated with sigma to phi* electronic transition of carbon in sucrose molecule. The absorbance at this UVC wavelength increased with increasing sucrose content. The quantitative analysis can be obtained by plotting a standard curve of absorbance versus Brix percentage. The results achieved in this work can be used to develop a low cost portable device for sucrose measurement using UVC lamp and photodiode as a light source and detector, respectively.
Keywords : % Brix: Sucrose Concentration: Beer-Lambert Law: UV-Vis Spectroscopy