8-10 June 2016
Asia/Bangkok timezone

Development of fluorescent-organically modified silica (ORMOSIL) nanoparticles as targeted probe for Leptospira

Jun 9, 2016, 12:15 PM
15m
Room Th

Room Th

Oral presentaion Material Physics, Nanoscale Physics and Nanotechnology Session XXII

Speakers

Ms Boonyaporn Suwattanapunkul (Student) Ms Jidapa Watthanaprakarnchai (student) Ms Naphatsorn Kulsayumporn (Student)

Description

Leptospirosis is an infectious disease caused by pathogens of the genus Leptospira. This

disease becomes a wide-spreading problem in tropical areas including Thailand. A method of

detection which is used nowadays is microscopic agglutination test (MAT) but its detection limit

is so high that it cannot be used to detect pathogens in low concentration samples. To develop

a new method that has lower detection limit, organically modified silica (ORMOSIL)

nanoparticles with encapsulated fluorescent dyes were used. In this research, fluoresecent-

ORMOSIL nanoparticles were synthesized and tagged by antibodies that are specific for

Leptospira. After synthesizing, these nanoparticles were characterized by transmission electron

microscope (TEM) which showed that they are spherical shapes and their average size is 42.4

nm. Moreover, they were analyzed by fourier transform infrared spectroscopy (FTIR) which

reported that there is carboxyl group on their surfaces. Finally, its detection limit is reported as

10 5 cells/ml by observing agglutination with pathogens under fluorescent microscope. According

to this experiment, we can conclude that fluorescent-ORMOSIL nanoparticles are able to be

used as a targeted probe for Leptospira. For future works, we expect to develop this method to

have a multiplex function and to be used as targeted probe for other diseases.

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