Speaker
Mrs
Germán Carrera
(Centro Nacional de Biotecnología CSIC)
Description
Bringing 3D-EM to the Grid.
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3D-EM is a well established technique that allows us to
visualize biological
structures across a wide range of sizes. As such, it
provides a bridge
between more fine-grained techniques (such as X-ray
crystallography or
NMR) and coarser methods like traditional light microscopy,
making
an un-substitutable tool for understanding subcellular
structures and
macromolecular assemblies. Its main downside being the heavy
demands on
compute power at some steps of the analytical process.
We have undertaken the project of bringing 3D-EM techniques
to the Grid.
Initial tests with 3D reconstruction yielded poor speedup
results turning
up important lessons for subsequent work. We are now dealing
with ML
classification of data, proceeding to refactor Xmipp and
dealing with
the major blockstoppers detected. Still, some relevant
issues worth noting
still remain that result in major conceptual challenges.
In this presentation we deal with the general problem of
3D-EM in its
experimental context, describe our approach, preliminary
results and
directions for future work.
Author
Mrs
Germán Carrera
(Centro Nacional de Biotecnología CSIC)
Co-authors
Alfredo Solano
Aristegui David García
Prof.
José-Maria Carazo
Dr
Valverde José R.
