Speaker
Mr
Supanat Sasipongpana
(College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand)
Description
A sensor for detecting pesticide by the inhibition of acetylcholine esterase (AChE) enzyme have been fabricated by direct immobilization in an agarose matrix on the surface of ion-sensitive field-effect transistors (ISFET). The enzyme entrapment was achieved by mixing AChE (0.01U) in an agarose gel buffer solution, and droping the mixture onto the surface of ISFET. This AChE-ISFET sensor was used to measure methyl-parathion in a phosphate buffer solution. The study of the agarose gel concentration (0.5%, 1.0%, 1.5% and 2.0%) and the storage time of sensor are reported. The device, which will be further explored in detecting other pesticides, is a promising pesticide sensor with simple preparation and capabilities to be developed as a portable device.
Primary author
Mr
Supanat Sasipongpana
(College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand)
Co-authors
Dr
Nongluck Houngkamhang
(College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand)
Dr
Pornpimol Sritongkham
(4Biomedical Engineering, Faculty of Engineering, Mahidol University, 999 Phuttamonthon 4 Road, Salaya, Nakhon Pathom 73170, Thailand)
Dr
Seeroong Prichanont
(Faculty of Engineering Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand)
Dr
Sirapat Pratontep
(College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand . ThEP Center, CHE, 328 Si Ayutthaya Rd., Bangkok 10400, Thailand)
Mr
Yossawat Rayanasukha
(College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand)
