20-22 May 2015
Asia/Bangkok timezone
The Centennial Celebration of General Relativity Theory and 80 Years of Thai Physics Graduate

AChE/agarose gel Coated on ISFET for Methyl-Parathion Sensors

20 May 2015, 14:45
15m
Phokeethra 1 ()

Phokeethra 1

Oral presentation Biological Physics and Biomedical Engineering Special Session: Pesticide Biosensor (Sponsored by MTEC)

Speaker

Mr Supanat Sasipongpana (College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand)

Description

A sensor for detecting pesticide by the inhibition of acetylcholine esterase (AChE) enzyme have been fabricated by direct immobilization in an agarose matrix on the surface of ion-sensitive field-effect transistors (ISFET). The enzyme entrapment was achieved by mixing AChE (0.01U) in an agarose gel buffer solution, and droping the mixture onto the surface of ISFET. This AChE-ISFET sensor was used to measure methyl-parathion in a phosphate buffer solution. The study of the agarose gel concentration (0.5%, 1.0%, 1.5% and 2.0%) and the storage time of sensor are reported. The device, which will be further explored in detecting other pesticides, is a promising pesticide sensor with simple preparation and capabilities to be developed as a portable device.

Primary author

Mr Supanat Sasipongpana (College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand)

Co-authors

Dr Nongluck Houngkamhang (College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand) Dr Pornpimol Sritongkham (4Biomedical Engineering, Faculty of Engineering, Mahidol University, 999 Phuttamonthon 4 Road, Salaya, Nakhon Pathom 73170, Thailand) Dr Seeroong Prichanont (Faculty of Engineering Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand) Dr Sirapat Pratontep (College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand . ThEP Center, CHE, 328 Si Ayutthaya Rd., Bangkok 10400, Thailand) Mr Yossawat Rayanasukha (College of Nanotechnology, King Mongkut’s Institute of Technology Ladkrabang, 1 Chalongkrung Rd., Ladkrabang, Bangkok 10520, Thailand)

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