Cryocoolers can experience near-periodic heaving or side-to-side motion and acceleration in some applications, in particular on moving platforms. In this work, the effect of heaving and side-to-side movement on a Stirling pulse tube cryocooler was experimentally studied. Periodic heaving and swaying motions having various amplitudes and frequencies were imposed on a Stirling pulse tube cryocooler, using a motion platform. The effects of the motions on the cooling performance of the Stirling pulse tube cryocooler were measured and analyzed. Reduction of cooling power were observed as a result of large acceleration and low frequency periodic motion. The results also indicated that the cryocooler is more sensitive to swaying motions than heaving motions.