Speaker
Description
Circular dichroism (CD) spectroscopy is a well-established tool in analytical biochemistry. In the deep-UV range (< 300 nm), it is sensitive to the spatial arrangement of transition dipoles on amino acid residues, nucleotides and peptides. Time-resolved CD spectroscopy is thus a promising experimental technique that is sensitive to changes in biomolecular configuration as a function of time, combining the time-dependent electronic information provided by traditional transient absorption spectroscopy with the structural information encoded in the chirality of molecular systems. In this context, we now report the first single-shot broadband circular dichroism spectrometer in the deep-UV (250–370 nm) with femtosecond time-resolution. Artefact-free static and transient CD spectra of enantiopure [Ru(bpy)$_3$]$^{2+}$ are successfully recorded at noise levels < 10$^{-5}$ OD.