Speaker
Description
Development of pesticide sensor based on the ion sensitive field effect transistor (ISFET) was investigated by using acetylcholinesterase which is an enzyme that catalyzes the hydrolysis of acetylcholine to acetate and choline. The ISFET can measure the inhibition reaction of acetylcholinesterase enzyme by the carbaryl pesticide through the pH changed in solution. The acetylcholinesterase was coated on ISFET’s surface by varying the enzyme concentration from 0.01 to 0.5 unit to select the optimum concentration for carbaryl detection. The pH effect of buffer solution was studied in the range of 6-10 to find the suitable pH for enzyme function which provides high sensitivity. The results showed that buffer solution pH 7 was the optimum pH for enzyme reaction and provided a good response for carbaryl detection in the range of 10$^{-6}$ to 10$^{-5}$ M. Moreover, the sensor stability was tested by comparison of the detection signal from ISFET that were kept at the different time.
