The Daya Bay Reactor Neutrino Experiment utilizes eight functionally identical detectors with large target volume to measure the antineutrino flux emitted from three pairs of powerful nuclear reactors at different baselines. The detectors are placed underground to provide enough shielding against cosmic rays induced backgrounds. The experiment can perform a high-statistics determination of the absolute reactor antineutrino flux and spectrum. It’s found that a deviation in the measured positron prompt energy spectrum with the comparison to model predictions. It’s observed a local discrepancy in the energy range of 4-6 MeV. The experiment gave the first measurement of individual spectra from 235U and 239Pu. This talk will report the latest measurements of antineutrino flux, spectral shape and evolution of these two quantities with nuclear fuel at Daya Bay.