The 16th International Conference on B-Physics at Frontier Machines, "Beauty 2016, took place in the Hôtel Mercure Vieux-Port, Marseille, France from 2nd to 6th May 2016. It was the 16th edition of the cycle “International Conference on B-Physics at Frontier Machines", initiated in Prague (Czech Republic) in 1993. The previous edition took place in Edinburgh in 2014. The aim of this conference was to review the latest theoretical and experimental results in heavy flavor physics. The scientific programme covered a wide range of researches including study of CP violation and rare decays properties of beauty and charm hadrons. This edition was particularly interesting since, amongst other, first results from the LHC were presented, following the LHC re-start in 2015 at an unprecedented colliding energy of 13TeV.