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This will be the 33rd meeting in this series of annual international multidisciplinary meetings, created in 1989 by Jean Trân Than Vân and which has in the past covered many topics in physics, astronomy and biology. All sessions take place in the Château of Blois, a beautiful renaissance castle which has housed many French kings, and notably François 1st. The 33rd Rencontres de Blois on "Exploring the Dark Universe" (Blois2022) review the most recent status of the field of Dark Matter, a lively and exciting scientific domain related to Cosmology, Astroparticles and Particle Physics. it will emphasize the big questions concerning the nature of Dark Matter and Dark Energy as seen both from high energy accelerator based physics and from deep underground experiments as well as from astrophysics and cosmology. The conference will consist of plenary sessions for invited in-depth oral presentations (review talks) and contributed papers, in the form of relatively short oral papers. We will aim to achieve a balance between review talks, provocative talks given by recognized specialists, and shorter contributions. Special emphasis is being placed on active participation by younger researchers and post-docs. Parallel sessions are foreseen, and are being organised as the need arises.